Microarrays can be used for transcriptional profiling, but also for genome wide fitness tests. Both methods aid in the identification of novel antibacterial modes of action.

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Transcriptomics

© Jem Stach - Material intended solely for students enrolled @ Newcastle University - MSc Industrial and Commercial Biotechnology. jem.stach@newcastle.ac.uk

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Freiberg C et al. Discovering the mechanism of action of novel antibacterial agents through transcriptional profiling of conditional mutants. Antimicrob Agents Chemother. 2005 Feb;49(2):749-59.

Xu D et al. Genome-wide fitness test and mechanism-of-action studies of inhibitory compounds in Candida albicans. PLoS Pathog. 2007 Jun;3(6):e92.

Brazas MD et al. Using microarray gene signatures to elucidate mechanisms of antibiotic action and resistance. Drug Discov Today. 2005 Sep 15;10(18):1245-52.

Ma H, & Horiuchi KY. Chemical microarray: a new tool for drug screening and discovery. Drug Discov Today. 2006 Jul;11(13-14):661-8.

LECTURE SEVEN