PRACTICAL

Manual

The practical makes up 60% of the assessment for this module

In this practical you will be repeating on a small scale the methods used in the early stages of antibiotic drug discovery: The genesis of 'Golden Age of Drug Discovery" was the identification of antimicrobial compounds from bacteria and fungi. The majority of novel antibiotics were discovered from the genus Streptomyces isolated from soil samples. Today ca. 70% of antibiotics on the market are derived from bacteria, many going to market unmodified! This practical aims to give you an appreciation of the initial stages of discovery: the isolation and screening of Streptomyces species for antibiotic activity.

Please read the practical instruction manual prior to coming to

the labs.

Background

© Jem Stach - Material intended solely for students enrolled @ Newcastle University - MSc Industrial and Commercial Biotechnology. jem.stach@newcastle.ac.uk

JS

Files

Tasks and Questionshttp://teaching.ncl.ac.uk/antimicrobials/Lectures/report_questions_2021.docx
Background of strain producing compound XBIO8041http://teaching.ncl.ac.uk/antimicrobials/Lectures/Details%20of%20the%20producer%20strain.pdf
Report writing guidelineshttp://teaching.ncl.ac.uk/antimicrobials/Lectures/report_writing_guidelines.doc
Background to Bacillus 
reporter strains 
http://teaching.ncl.ac.uk/antimicrobials/Lectures/Bacillus_reporter_strain_overview.pdf
Positive controls for 
Bacillus reportershttp://teaching.ncl.ac.uk/antimicrobials/Lectures/reporter_controls.jpg
Group screening resultshttp://teaching.ncl.ac.uk./antimicrobials/Lectures/group_screening_data.pdf
../Site/Forum.html
../Site/Home.html
http://teaching.ncl.ac.uk/antimicrobials/Lectures/BIO8041%20manual%202019.pdf
BIO8041http://teaching.ncl.ac.uk/antimicrobials/Lectures/BIO8041%20manual%202019.pdf
PRACTICALhttp://teaching.ncl.ac.uk/antimicrobials/Lectures/BIO8041%20manual%202019.pdf