https://teaching.ncl.ac.uk/bms/wiki//api.php?action=feedcontributions&feedformat=atom&user=050272646The School of Biomedical Sciences Wiki - User contributions [en]2026-04-14T23:53:08ZUser contributionsMediaWiki 1.44.0https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Asparagine&diff=799Asparagine2010-11-13T16:24:59Z<p>050272646: Created page with 'Asparagine is a non-essential amino acid. Its abbreviations are Asn or N, and its codons are AAU or AAC. It provides an important site for asparagine-linked glycosylation (N-gl…'</p>
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<div>Asparagine is a non-essential amino acid. Its abbreviations are Asn or N, and its codons are AAU or AAC. <br />
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It provides an important site for asparagine-linked glycosylation (N-glycosylation) within peptides.<br />
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'''<u>Synthesis</u>'''<br />
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'''&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; '''(transaminase)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (asparagine synthase)<br />
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Oxaloacetate&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;⇒&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Aspartate&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;⇒&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Asparagine<br />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;glutamate&nbsp;&nbsp;&nbsp;→&nbsp; alpha-ketoglutarate&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;glutamine&nbsp;&nbsp;→&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;glutamate<br />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ATP&nbsp;&nbsp; →&nbsp;&nbsp;AMP + PPi<br />
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<u>References</u><br />
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Kenneth W. Joy, Robert J. Ireland and Peter J. Lea, 'Asparagine Synthesis in Pea Leaves, and the Occurrence of an Asparagine Synthetase Inhibitor' Plant Physiology (1983)&nbsp;73:165-168</div>050272646https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Assay&diff=543Assay2010-11-11T10:47:15Z<p>050272646: </p>
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<div>Measure. So an [[Enzyme|enzyme]] assay is a procedure to measure the activity of the [[Enzyme|enzyme]]. E.g. A dual reporter assay measures luciferase and B-galactosidase activity. These are both [[Enzyme|enzymes]].</div>050272646