The School of Biomedical Sciences Wiki - User contributions [en]

Non-denaturing gel

2011-12-02T09:24:52Z

110027656:

Non-denaturing gel

2011-12-02T09:20:51Z

110027656:

Non-denaturing gel

2011-12-02T09:20:02Z

110027656:

Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse [[cell|cell]] membranes and no charged denaturing agents are used. The molecules being separated remain folded<ref>http://www.ap-lab.com/native_gels.htm</ref>, however separation does not occur as predictibly as those run in a denaturing gel.

<span style="font-weight: bold;" />Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis (PAGE). SDS is not used and detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded[1], however separation does not occur as predictibly as those run in a denaturing gel, as shape does not usually relate to  size and charge may not be as evenly distributed compared to proteins run in [[SDS_polyacrylamide-gel_electrophoresis]].

Non-denaturing gel

2011-11-26T15:07:11Z

110027656:

Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded<ref>http://www.ap-lab.com/native_gels.htm</ref>, however separation does not occur as predictibly as those run in a denaturing gel.

<references />

Non-denaturing gel

2011-11-26T14:59:26Z

110027656:

Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded, however separation does not occur as predictibly as those run in a denaturing gel.

<references />[http://www.ap-lab.com/native_gels.htm http://www.ap-lab.com/native_gels.htm]

Non-denaturing gel

2011-11-26T14:54:26Z

110027656: Created page with "Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis (PAGE). Detergents ..."

Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis ([[SDS_polyacrylamide-gel_electrophoresis|PAGE]]). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded, however separation does not occur as predictibly as those run in a denaturing gel.