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Nitric oxide

2015-10-20T14:28:48Z

130070140:

Nitric oxide is a chemical compound made up of 1 atom of Nitrogen and 1 atom of oxygen with the chemical formula NO<ref>https://en.wikipedia.org/wiki/Nitric_oxide</ref>. It said to be important in many cellular activities including memory and regulation of blood pressure<ref>http://www.nutritionexpress.com/showarticle.aspx?articleid=286</ref>.

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Nitric oxide

2015-10-20T14:28:17Z

130070140: Created page with "Nitric oxide is a chemical compound made up of 1 atom of Nitrogen and 1 atom of oxygen with the chemical formula NO<ref name="1">https://en.wikipedia.org/wiki/Nitric_oxide</ref>...."

Nitric oxide is a chemical compound made up of 1 atom of Nitrogen and 1 atom of oxygen with the chemical formula NO<ref name="1">https://en.wikipedia.org/wiki/Nitric_oxide</ref>. It said to be important in many cellular activities including memory and regulation of blood pressure<ref>http://www.nutritionexpress.com/showarticle.aspx?articleid=286</ref>.

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Imac chromatography

2014-11-27T17:42:29Z

130070140: Created page with " IMAC - Immobilised metal affinity chromatography is one type of column chromatography used to seperate protein/amino acids from a mixture. This method of chromatography req..."

 IMAC - Immobilised metal affinity chromatography is one type of column chromatography used to seperate protein/amino acids from a mixture. This method of chromatography requires recombinant protein expression: the natural DNA sequence for a protein is taken and is engineered to include 6-10 histidine residues at the N or C terminus of a protein. This is nicknamed the 'His-Tag'. Genetic engineering is useed to produce a protein in a host such as bacteria. Histidine is a charged heteroaromati amino acid with a imidazole side chain which can bind to metals such as Nickel or Zinc. To seperate proteins not containing the His-Tag in a chromatography column, the mixture is ran down the column and all proteins containing a his-tag will elute out of the column. Once all other proteins have eluted, to then remove the desired proteins containing the His-Tag, you then add imidazole which is very similar to histidine. The imidazole will compete with the histidine to bind with the Nickel or Zincm but has a higher affinitiy than the histidine so seperates it from the metal ions, allowing it to elute out of the column.<ref>http://www2.knauer.net/dwnld_fls/b_e_co_biofox_imac_tren_ida_17+40.pdf</ref>

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