Dideoxy sequencing

2018-12-10T00:16:34Z

180172379: Created page with "Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the s..."

Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the sequence of neuclotides in DNA<ref>This is a reference to the website AK lectures which also has links to the YouTube video i also used.
http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the incorperation of the meolecule  2',3'-dideoxynucleoside triphosphate(ddNTP).This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>.This stops DNA replication.

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Dideoxy sequencing