https://teaching.ncl.ac.uk/bms/wiki//index.php?action=history&feed=atom&title=%27sticky%27_ends'sticky' ends - Revision history2026-04-10T18:13:31ZRevision history for this page on the wikiMediaWiki 1.44.0https://teaching.ncl.ac.uk/bms/wiki//index.php?title=%27sticky%27_ends&diff=22652&oldid=prevNnjm2: Added some links.2018-12-04T18:38:25Z<p>Added some links.</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 18:38, 4 December 2018</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">&nbsp;</del>Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as&nbsp;EcoRI. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by DNA ligase which catalyses the formation of bonds between the sugar-phosphate groups between the fragments<sup><ref>Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.</ref></sup><sup></sup>. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Also known as cohesive ends, sticky ends are formed when a double stranded <ins style="font-weight: bold; text-decoration: none;">[[</ins>DNA<ins style="font-weight: bold; text-decoration: none;">|DNA]] </ins>fragment is asymmetrically cleaved by certain <ins style="font-weight: bold; text-decoration: none;">[[</ins>restriction enzymes<ins style="font-weight: bold; text-decoration: none;">|restriction enzymes]] </ins>such as&nbsp;<ins style="font-weight: bold; text-decoration: none;">[[</ins>EcoRI<ins style="font-weight: bold; text-decoration: none;">|EcoRI]]</ins>. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by <ins style="font-weight: bold; text-decoration: none;">[[</ins>DNA ligase<ins style="font-weight: bold; text-decoration: none;">|DNA ligase]] </ins>which catalyses the formation of bonds between the <ins style="font-weight: bold; text-decoration: none;">[[sugar-phosphate groups|</ins>sugar-phosphate groups<ins style="font-weight: bold; text-decoration: none;">]] </ins>between the fragments<sup><ref>Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.</ref></sup><sup></sup>. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>This technique is often used in recombinant DNA technology. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>This technique is often used in <ins style="font-weight: bold; text-decoration: none;">[[</ins>recombinant DNA technology<ins style="font-weight: bold; text-decoration: none;">|recombinant DNA technology]]</ins>.<ins style="font-weight: bold; text-decoration: none;"><br> </ins></div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"><br> </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=== References<del style="font-weight: bold; text-decoration: none;">: </del>===</div></td><td colspan="2" class="diff-side-added"></td></tr>
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</table>Nnjm2https://teaching.ncl.ac.uk/bms/wiki//index.php?title=%27sticky%27_ends&diff=22617&oldid=prev170071488: Created page with " Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as EcoRI. These e..."2018-12-04T17:00:33Z<p>Created page with " Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as EcoRI. These e..."</p>
<p><b>New page</b></p><div>&nbsp;Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as&nbsp;EcoRI. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by DNA ligase which catalyses the formation of bonds between the sugar-phosphate groups between the fragments<sup><ref>Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.</ref></sup><sup></sup>. <br />
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This technique is often used in recombinant DNA technology. <br />
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=== References: ===<br />
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