Nnjm2 at 09:29, 10 December 2018

2018-12-10T09:29:33Z

← Older revision		Revision as of 09:29, 10 December 2018
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	Dideoxy sequencing,also known as the chain terminating method or ~~sanger~~ sequencing , is a DNA ~~aequencing~~ method created by Biochemist Fred Sanger and is used in determining the sequence of ~~neuclotides~~ in DNA<ref>This is a reference to the website AK lectures which also has links to the YouTube video i also used.		Dideoxy sequencing, also known as the chain terminating method or [[Sanger_Method\|Sanger]] sequencing, is a [[DNA\|DNA]] sequencing method created by Biochemist [[Frederick_Sanger\|Fred Sanger]] and is used in determining the sequence of [[nucleotides\|nucleotides]] in [[DNA\|DNA]]<ref>This is a reference to the website AK lectures which also has links to the YouTube video I also used. http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the incorporation of the [[molecule\|molecule]] [[ddNTP\|2',3'-dideoxynucleoside triphosphate]] ([[ddNTP\|ddNTP]]). This molecule lacks a [[hydroxyl group\|hydroxyl group]] so [[DNA polymerase\|DNA polymerase]] can no longer form a [[phosphodiester bond\|phosphodiester bond]]<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>. This stops DNA replication.
	http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the ~~incorperation~~ of the ~~meolecule ~~ 2',3'-dideoxynucleoside triphosphate(ddNTP).This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>.This stops DNA replication.

	~~'''~~References~~'''~~		=== References ===

	~~'''~~<references />~~'''~~		<references />

180172379: Created page with "Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the s..."

2018-12-10T00:16:34Z

Created page with "Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the s..."

New page

Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the sequence of neuclotides in DNA<ref>This is a reference to the website AK lectures which also has links to the YouTube video i also used.
http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the incorperation of the meolecule  2',3'-dideoxynucleoside triphosphate(ddNTP).This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>.This stops DNA replication.

'''References'''

'''<references />'''

Dideoxy sequencing - Revision history

Nnjm2 at 09:29, 10 December 2018

180172379: Created page with "Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the s..."