Electrophoresis - Revision history

https://teaching.ncl.ac.uk/bms/wiki//index.php?action=history&feed=atom&title=Electrophoresis Electrophoresis - Revision history 2026-04-08T21:36:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=16697&oldid=prev 160154980 at 17:08, 4 December 2016 2016-12-04T17:08:56Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:08, 4 December 2016</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter <del style="font-weight: bold; text-decoration: none;">(usually [[</del>Polyacrylamide<del style="font-weight: bold; text-decoration: none;">|polyacylamide]] </del>gels are used<del style="font-weight: bold; text-decoration: none;">)</del>. There are two separate portions presence across the gel, stacking gel and resolving gel. The stacking gel consists low percentage of polyacrylamide where bigger molecule tends to move slower. While the resolving gel contains higher percentage of polyacrylamide, which the smaller molecule can move faster and further in distance. &nbsp;By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter<ins style="font-weight: bold; text-decoration: none;">. Gels are used to prevent convection currents. </ins>Polyacrylamide gels are used <ins style="font-weight: bold; text-decoration: none;">and separates proteins depending on charge, size and shape. The speed of the migration of the macromolecules is also influenced by the gel's pore size</ins>. There are two separate portions presence across the gel, stacking gel and resolving gel. The stacking gel consists low percentage of polyacrylamide where bigger molecule tends to move slower. While the resolving gel contains higher percentage of polyacrylamide, which the smaller molecule can move faster and further in distance. &nbsp;By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by: </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by: </div></td></tr> </table>

160154980 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=10674&oldid=prev 140001613 at 15:28, 9 October 2014 2014-10-09T15:28:56Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:28, 9 October 2014</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). <ins style="font-weight: bold; text-decoration: none;">There are two separate portions presence across the gel, stacking gel and resolving gel. The stacking gel consists low percentage of polyacrylamide where bigger molecule tends to move slower. While the resolving gel contains higher percentage of polyacrylamide, which the smaller molecule can move faster and further in distance. &nbsp;</ins>By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by: </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by: </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>*Net Charge of molecule. Negitive charged molecules move towards the positive end of the electrode (anode) while positive charged molecule move towards the negitive end of electrode (cathode).&nbsp;</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>*Net Charge of molecule. Negitive charged molecules move towards the positive end of the electrode (anode) while positive charged molecule move towards the negitive end of electrode (cathode).&nbsp; </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>*Size of [[<del style="font-weight: bold; text-decoration: none;">molecule</del>|molecule]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>*Size of [[<ins style="font-weight: bold; text-decoration: none;">Molecule</ins>|molecule]] </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>*Shape</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>*Shape </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Electrical field strength applied<ref>Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Electrical field strength applied<ref>Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited</ref></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>=== References <ins style="font-weight: bold; text-decoration: none;"> </ins>===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

140001613 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=7749&oldid=prev Nnjm2 at 16:27, 29 November 2012 2012-11-29T16:27:56Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:27, 29 November 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by:</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The mobility of charges molecules are influenced by: </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">-</del>Net Charge of molecule. Negitive charged molecules move towards the positive end of the electrode (anode) while positive charged molecule move towards the negitive end of electrode (cathode).&nbsp;</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*</ins>Net Charge of molecule. Negitive charged molecules move towards the positive end of the electrode (anode) while positive charged molecule move towards the negitive end of electrode (cathode).&nbsp;</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*Size of [[molecule|molecule]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*Shape</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*Electrical field strength applied<ref>Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited</ref></ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">-Size of molecule</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== References ===</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">-Shape-</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">-Electrical field strength applied</del><references /><del style="font-weight: bold; text-decoration: none;">&nbsp;Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited&nbsp;</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table>

Nnjm2 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=7682&oldid=prev 120332926 at 12:51, 29 November 2012 2012-11-29T12:51:44Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 12:51, 29 November 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter (usually [[Polyacrylamide|polyacylamide]] gels are used). By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">The mobility of charges molecules are influenced by:</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">-Net Charge of molecule. Negitive charged molecules move towards the positive end of the electrode (anode) while positive charged molecule move towards the negitive end of electrode (cathode).&nbsp;</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">-Size of molecule</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">-Shape-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">-Electrical field strength applied<references />&nbsp;Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited&nbsp;</ins></div></td></tr> </table>

120332926 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=4965&oldid=prev 110027564 at 15:19, 1 December 2011 2011-12-01T15:19:46Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:19, 1 December 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter <ins style="font-weight: bold; text-decoration: none;">(usually [[Polyacrylamide|polyacylamide]] gels are used)</ins>. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br></div></td></tr> </table>

110027564 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=4963&oldid=prev 110027564 at 15:17, 1 December 2011 2011-12-01T15:17:09Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:17, 1 December 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. <ins style="font-weight: bold; text-decoration: none;">Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br></ins></div></td></tr> </table>

110027564 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=2781&oldid=prev Nnjm2 at 17:36, 10 January 2011 2011-01-10T17:36:16Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:36, 10 January 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[<del style="font-weight: bold; text-decoration: none;">SDS_polyacrylamide</del>-<del style="font-weight: bold; text-decoration: none;">gel_electrophoresis</del>|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[<ins style="font-weight: bold; text-decoration: none;">SDS polyacrylamide</ins>-<ins style="font-weight: bold; text-decoration: none;">gel electrophoresis</ins>|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td></tr> </table>

Nnjm2 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=2748&oldid=prev 100524996 at 16:54, 10 January 2011 2011-01-10T16:54:07Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:54, 10 January 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Electrophoresis is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[SDS_polyacrylamide-gel_electrophoresis|</ins>Electrophoresis<ins style="font-weight: bold; text-decoration: none;">]] </ins>is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td></tr> </table>

100524996 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=1602&oldid=prev Nnjm2 at 19:05, 23 November 2010 2010-11-23T19:05:39Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:05, 23 November 2010</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Electrophoresis is used as a method of separating<del style="font-weight: bold; text-decoration: none;">&nbsp;</del>[<del style="font-weight: bold; text-decoration: none;">http://bms.ncl.ac.uk/wiki/index.php/</del>DNA DNA]<del style="font-weight: bold; text-decoration: none;">&nbsp;</del>according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Electrophoresis is used as a method of separating [<ins style="font-weight: bold; text-decoration: none;">[</ins>DNA<ins style="font-weight: bold; text-decoration: none;">|</ins>DNA]<ins style="font-weight: bold; text-decoration: none;">] </ins>according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td></tr> </table>

Nnjm2 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Electrophoresis&diff=1575&oldid=prev 090331619 at 15:58, 23 November 2010 2010-11-23T15:58:16Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:58, 23 November 2010</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Electrophoresis is used as a method of separating&nbsp;[http://bms.ncl.ac.uk/wiki/<del style="font-weight: bold; text-decoration: none;">extensions/FCKeditor/fckeditor/editor</del>/DNA DNA]&nbsp;according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Electrophoresis is used as a method of separating&nbsp;[http://bms.ncl.ac.uk/wiki/<ins style="font-weight: bold; text-decoration: none;">index.php</ins>/DNA DNA]&nbsp;according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously.</div></td></tr> </table>

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