Okazaki fragments - Revision history

150015938 at 08:50, 17 November 2014

2014-11-17T08:50:04Z

← Older revision		Revision as of 08:50, 17 November 2014
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	During [[DNA replication\|DNA replication]] the [[DNA\|DNA]] duplex is unwound and two daughter strands are formed at the [[~~replication~~ fork\|replication fork]]. [[DNA polymerase\|DNA polymerase]] can only add [[~~nucleotide~~\|nucleotides]] in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.		During [[DNA replication\|DNA replication]] the [[DNA\|DNA]] duplex is unwound and two daughter strands are formed at the [[Replication fork\|replication fork]]. [[DNA polymerase\|DNA polymerase]] can only add [[Nucleotide\|nucleotides]] in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the [[Replication_fork\|replication fork]].

	However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new [[RNA\|RNA]] primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer [[Reiji Okazaki\|Reiji Okazaki]]. The RNA primer is then removed and the [[~~enzyme~~\|enzyme]] [[DNA ligase\|DNA ligase]] joins the adjacent fragments together <ref name="null">Molecular Cell Biology, 7th Edtion, Harvey Lodish et al., Page: 146</ref>.		However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new [[RNA\|RNA]] primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer [[Reiji Okazaki\|Reiji Okazaki]]. The RNA primer is then removed and the [[Enzyme\|enzyme]] [[DNA ligase\|DNA ligase]] joins the adjacent fragments together <ref name="null">Molecular Cell Biology, 7th Edtion, Harvey Lodish et al., Page: 146</ref>.

	=== References ===		=== References ===

	<references />		<references />

Nnjm2 at 16:26, 29 November 2012

2012-11-29T16:26:58Z

← Older revision		Revision as of 16:26, 29 November 2012
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	During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.		During [[DNA replication\|DNA replication]] the [[DNA\|DNA]] duplex is unwound and two daughter strands are formed at the [[replication fork\|replication fork]]. [[DNA polymerase\|DNA polymerase]] can only add [[nucleotide\|nucleotides]] in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.

	However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.<ref name="null">Molecular Cell Biology, 7th Edtion, Harvey Lodish et al., Page: 146</ref>		However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new [[RNA\|RNA]] primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer [[Reiji Okazaki\|Reiji Okazaki]]. The RNA primer is then removed and the [[enzyme\|enzyme]] [[DNA ligase\|DNA ligase]] joins the adjacent fragments together <ref name="null">Molecular Cell Biology, 7th Edtion, Harvey Lodish et al., Page: 146</ref>.

	=== References ===		=== References ===

	<references />		<references />

110026626 at 12:56, 29 November 2012

2012-11-29T12:56:56Z

← Older revision		Revision as of 12:56, 29 November 2012
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	During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.		During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.

	However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.<ref>~~Pg. 146,~~ Molecular Cell Biology, Harvey Lodish et al.</ref>		However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.<ref name="null">Molecular Cell Biology, 7th Edtion, Harvey Lodish et al., Page: 146</ref>

	=== References ===		=== References ===

	<references />		<references />

110026626 at 12:55, 29 November 2012

2012-11-29T12:55:31Z

← Older revision		Revision as of 12:55, 29 November 2012
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	During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.		During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.

	However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.		However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.<ref>Pg. 146, Molecular Cell Biology, Harvey Lodish et al.</ref>

			=== References ===

			<references />


	~~=== References ===~~

	~~<ref>Pg. 146, Molecular Cell Biology, Harvey Lodish et al.</ref>~~<references />

110026626 at 12:54, 29 November 2012

2012-11-29T12:54:52Z

← Older revision		Revision as of 12:54, 29 November 2012
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	During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.		During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.

	However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.~~<references />~~<ref>~~Lodish, H~~. ~~et al~~,~~(2012)~~ Molecular Cell Biology, ~~7th edition, pg~~. ~~146~~</ref>		However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.





			=== References ===

			<ref>Pg. 146, Molecular Cell Biology, Harvey Lodish et al.</ref><references />

110026626: Created page with "During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, h..."

2012-11-29T12:51:16Z

Created page with "During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, h..."

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During DNA replication the DNA duplex is unwound and two daughter strands are formed at the replication fork. DNA polymerase can only add nucleotides in the 5' to 3' direction, hence the synthesis of the one of the daughter strands, the leading strand, can proceed continuously in the same direction as movement of the replication fork.

However a complication arises with the replication of the other daughter strand, the lagging strand. As growth must occur in the 5' to 3' direction the copying of the template must somehow occur in the opposite direction as the movement of the replication fork. The cell accomplishes this by synthesising a new RNA primer every hundred bases or so as more of the strand is exposed. Each of these primers is elongated in the 5' to 3' direction forming segments called Okazaki fragments, named after their discoverer Reiji Okazaki. The RNA primer is then removed and the enzyme DNA ligase joins the adjacent fragments together.<references /><ref>Lodish, H. et al,(2012) Molecular Cell Biology, 7th edition, pg. 146</ref> 