Rolling circle replication - Revision history

Nnjm2 at 20:04, 5 December 2017

2017-12-05T20:04:21Z

← Older revision		Revision as of 20:04, 5 December 2017
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	[[Image:RollingCircleReplication.jpg\|~~center~~\|400px~~\|right~~\|Mechanism of rolling circle replication in DNA]] Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.		[[Image:RollingCircleReplication.jpg\|right\|400px\|Mechanism of rolling circle replication in DNA]] Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.

	=== Rolling Circle Replication Mechanism in DNA ===		=== Rolling Circle Replication Mechanism in DNA ===

Nnjm2 at 20:04, 5 December 2017

2017-12-05T20:04:06Z

← Older revision		Revision as of 20:04, 5 December 2017
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	[[Image:RollingCircleReplication.jpg\|center\|400px\|Mechanism of rolling circle replication in DNA~~\|right~~]]		[[Image:RollingCircleReplication.jpg\|center\|400px\|right\|Mechanism of rolling circle replication in DNA]] Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.
	Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.

	=== Rolling Circle Replication Mechanism in DNA ===		=== Rolling Circle Replication Mechanism in DNA ===

	The process of DNA is initiated by initiator protein which nicks at the site called the double-stranded origin on one strand of the double-strand. The initiator protein remains on the 5’ phosphate nick strand, and the 3’ hydroxyl end of the nicked strand is elongated by DNA polymerase III. The unnicked strand acts as the template strand for replication and the 5’ phosphate nick strand is displace by helicase. Eventually, the nick strand is completely displaced by newly synthesised strand and will remove itself from the original circular DNA by the same initiator protein nicking at the terminating sequence on the nicked strand. The nicked strand then form a new single-stranded circular DNA molecule. RNA polymerase and DNA polymerase III then use the single strand as a template to form new double-stranded circular DNA molecule<ref>Ruiz-Masó J, MachóN C, Bordanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. Plasmid rolling-circle replication. microbial spectrum. 2015;3;10.1128</ref>.		The process of DNA is initiated by initiator protein which nicks at the site called the double-stranded origin on one strand of the double-strand. The initiator protein remains on the 5’ phosphate nick strand, and the 3’ hydroxyl end of the nicked strand is elongated by DNA polymerase III. The unnicked strand acts as the template strand for replication and the 5’ phosphate nick strand is displace by helicase. Eventually, the nick strand is completely displaced by newly synthesised strand and will remove itself from the original circular DNA by the same initiator protein nicking at the terminating sequence on the nicked strand. The nicked strand then form a new single-stranded circular DNA molecule. RNA polymerase and DNA polymerase III then use the single strand as a template to form new double-stranded circular DNA molecule<ref>Ruiz-Masó J, MachóN C, Bordanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. Plasmid rolling-circle replication. microbial spectrum. 2015;3;10.1128</ref>.

	=== References ===		=== References ===

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Nnjm2: Added the references correctly, that is, I added them as explained in the lecture. Reformatted the page. Cleaned up the text.

2017-12-05T20:03:45Z

Added the references correctly, that is, I added them as explained in the lecture. Reformatted the page. Cleaned up the text.

← Older revision		Revision as of 20:03, 5 December 2017
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	~~ ~~Rolling circle~~ ~~replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.~~ ~~		[[Image:RollingCircleReplication.jpg\|center\|400px\|Mechanism of rolling circle replication in DNA\|right]]
			Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome.

	= ~~ ~~ Rolling Circle Replication Mechanism in DNA =		=== Rolling Circle Replication Mechanism in DNA ===

	The process in DNA is initiated by initiator protein which nicks at the site called the double stranded origin on one strand of the double-strand. The initiator protein remains on the 5’ phosphate nick strand, and the 3’ hydroxyl end of the ~~nick~~ strand is elongated by DNA polymerase III. The unnicked strand acts as the template strand for replication and the 5’ phosphate nick strand is displace by helicase. Eventually, the nick strand is completely displaced by newly ~~synthesis~~ strand and will remove itself from the original circular DNA by the same initiator protein nicking at the terminating sequence on the ~~nick~~ strand. The ~~nick~~ strand then form a new single stranded circular DNA molecule. RNA polymerase and DNA polymerase III then use the single strand as template to form new double stranded circular DNA molecule<ref>Ruiz-Masó J, MachóN C, Bordanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. Plasmid rolling-circle replication. ~~microbiolspectrum~~. 2015;3;10.1128</ref>.~~ ~~		The process of DNA is initiated by initiator protein which nicks at the site called the double-stranded origin on one strand of the double-strand. The initiator protein remains on the 5’ phosphate nick strand, and the 3’ hydroxyl end of the nicked strand is elongated by DNA polymerase III. The unnicked strand acts as the template strand for replication and the 5’ phosphate nick strand is displace by helicase. Eventually, the nick strand is completely displaced by newly synthesised strand and will remove itself from the original circular DNA by the same initiator protein nicking at the terminating sequence on the nicked strand. The nicked strand then form a new single-stranded circular DNA molecule. RNA polymerase and DNA polymerase III then use the single strand as a template to form new double-stranded circular DNA molecule<ref>Ruiz-Masó J, MachóN C, Bordanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. Plasmid rolling-circle replication. microbial spectrum. 2015;3;10.1128</ref>.

	~~<br>~~		=== References ===

	~~[[Image:RollingCircleReplication.jpg\|center\|700px\|Mechanism of rolling circle replication in DNA]]<br>~~

	~~<br>~~

	<references />		<references />

170085463: Created page with " Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s ge..."

2017-12-05T17:17:05Z

Created page with " Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s ge..."

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 Rolling circle replication is a process which a circular DNA or RNA molecule is replicated in one direction. This particular process occurs in plasmid and virus’s genome. 

=   Rolling Circle Replication Mechanism in DNA =

The process in DNA is initiated by initiator protein which nicks at the site called the double stranded origin on one strand of the double-strand. The initiator protein remains on the 5’ phosphate nick strand, and the 3’ hydroxyl end of the nick strand is elongated by DNA polymerase III. The unnicked strand acts as the template strand for replication and the 5’ phosphate nick strand is displace by helicase. Eventually, the nick strand is completely displaced by newly synthesis strand and will remove itself from the original circular DNA by the same initiator protein nicking at the terminating sequence on the nick strand. The nick strand then form a new single stranded circular DNA molecule. RNA polymerase and DNA polymerase III then use the single strand as template to form new double stranded circular DNA molecule<ref>Ruiz-Masó J, MachóN C, Bordanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. Plasmid rolling-circle replication. microbiolspectrum. 2015;3;10.1128</ref>. 

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[[Image:RollingCircleReplication.jpg|center|700px|Mechanism of rolling circle replication in DNA]]<br>

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