SDS polyacrylamide-gel electrophoresis - Revision history

https://teaching.ncl.ac.uk/bms/wiki//index.php?action=history&feed=atom&title=SDS_polyacrylamide-gel_electrophoresis SDS polyacrylamide-gel electrophoresis - Revision history 2026-04-08T21:37:02Z Revision history for this page on the wiki MediaWiki 1.44.0 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=18226&oldid=prev Nnjm2 at 17:44, 25 October 2017 2017-10-25T17:44:36Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:44, 25 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to <del style="font-weight: bold; text-decoration: none;">seperate </del>[[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size<ref>Rath A, Glibowicka M, Nadeau VG, Chen G, Deber CM., Detergent binding explains anomalous SDS-PAGE migration of membrane proteins., 2009 Feb 10;106(6):1760-5. Available at: http://www.pnas.org/content/106/6/1760.full#aff-2</ref>. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate <del style="font-weight: bold; text-decoration: none;">towrds </del>the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for [[Membrane proteins|membrane proteins]] which are typically insoluble in water due to the <del style="font-weight: bold; text-decoration: none;">seperation occuring </del>as a result of the size of the [[Polypeptide chain|polypeptide chain]]<ref>cell. 2006</ref>. Dyes such as [[Coomassie blue|Coomassie Blue]] are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to <ins style="font-weight: bold; text-decoration: none;">separate </ins>[[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size<ref>Rath A, Glibowicka M, Nadeau VG, Chen G, Deber CM., Detergent binding explains anomalous SDS-PAGE migration of membrane proteins., 2009 Feb 10;106(6):1760-5. Available at: http://www.pnas.org/content/106/6/1760.full#aff-2</ref>. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate <ins style="font-weight: bold; text-decoration: none;">towards </ins>the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for [[Membrane proteins|membrane proteins]] which are typically insoluble in water due to the <ins style="font-weight: bold; text-decoration: none;">separation occurring </ins>as a result of the size of the [[Polypeptide chain|polypeptide chain]]<ref>cell. 2006</ref>. Dyes such as [[Coomassie blue|Coomassie Blue]] are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"><span style</del>=<del style="font-weight: bold; text-decoration: none;">"font-size: 17.529600143432617px; font-weight: bold;"></del>References<del style="font-weight: bold; text-decoration: none;"></span> </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>=<ins style="font-weight: bold; text-decoration: none;">== </ins>References <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

Nnjm2 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=18216&oldid=prev 160234846 at 17:13, 25 October 2017 2017-10-25T17:13:01Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:13, 25 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for membrane proteins which are typically insoluble in water due to the seperation occuring as a result of the size of the polypeptide chain<ref>cell. 2006</ref>. Dyes such as Coomassie Blue are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size<ins style="font-weight: bold; text-decoration: none;"><ref>Rath A, Glibowicka M, Nadeau VG, Chen G, Deber CM., Detergent binding explains anomalous SDS-PAGE migration of membrane proteins., 2009 Feb 10;106(6):1760-5. Available at: http://www.pnas.org/content/106/6/1760.full#aff-2</ref></ins>. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for <ins style="font-weight: bold; text-decoration: none;">[[Membrane proteins|</ins>membrane proteins<ins style="font-weight: bold; text-decoration: none;">]] </ins>which are typically insoluble in water due to the seperation occuring as a result of the size of the <ins style="font-weight: bold; text-decoration: none;">[[Polypeptide chain|</ins>polypeptide chain<ins style="font-weight: bold; text-decoration: none;">]]</ins><ref>cell. 2006</ref>. Dyes such as <ins style="font-weight: bold; text-decoration: none;">[[Coomassie blue|</ins>Coomassie Blue<ins style="font-weight: bold; text-decoration: none;">]] </ins>are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=<del style="font-weight: bold; text-decoration: none;">== </del>References <del style="font-weight: bold; text-decoration: none;"> ===</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><span style</ins>=<ins style="font-weight: bold; text-decoration: none;">"font-size: 17.529600143432617px; font-weight: bold;"></ins>References<ins style="font-weight: bold; text-decoration: none;"></span> </ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

160234846 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=13825&oldid=prev 140418268 at 11:24, 23 October 2015 2015-10-23T11:24:25Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:24, 23 October 2015</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for membrane proteins which are typically insoluble in water due to the seperation occuring as a result of the size of the polypeptide chain. Dyes such as Coomassie Blue are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. This method can also be used for membrane proteins which are typically insoluble in water due to the seperation occuring as a result of the size of the polypeptide chain<ins style="font-weight: bold; text-decoration: none;"><ref>cell. 2006</ref></ins>. Dyes such as Coomassie Blue are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

140418268 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=9508&oldid=prev 130024187 at 13:06, 15 November 2013 2013-11-15T13:06:40Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:06, 15 November 2013</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.<del style="font-weight: bold; text-decoration: none;">&nbsp; </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and size. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate towrds the positive end when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. <ins style="font-weight: bold; text-decoration: none;">This method can also be used for membrane proteins which are typically insoluble in water due to the seperation occuring as a result of the size of the polypeptide chain. Dyes such as Coomassie Blue are used to stain and identify major proteins whereas silver or gold stain is used to highlight smaller proteins<ref>Alberts et al., Molecular Biology of the Cell, 2008, 5th Ed., Pg 517</ref>. </ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

130024187 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=7584&oldid=prev 110559490 at 11:39, 29 November 2012 2012-11-29T11:39:33Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:39, 29 November 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and <del style="font-weight: bold; text-decoration: none;">shape</del>. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate <del style="font-weight: bold; text-decoration: none;">through </del>when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.&nbsp;</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and <ins style="font-weight: bold; text-decoration: none;">size</ins>. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate <ins style="font-weight: bold; text-decoration: none;">towrds the positive end </ins>when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.&nbsp; </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

110559490 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=6517&oldid=prev Nnjm2 at 21:31, 22 October 2012 2012-10-22T21:31:59Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:31, 22 October 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.<del style="font-weight: bold; text-decoration: none;">Once an SDS-Page Gel electrophoresis has taken place, the fragments of [[DNA|DNA ]]then need to be visualised. This can be done through a few different methods. One way is through UV imaging, another is through adding a dye to the [[DNA|DNA]] fragments. </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.<ins style="font-weight: bold; text-decoration: none;">&nbsp;</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

Nnjm2 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=6514&oldid=prev 110254403 at 21:27, 22 October 2012 2012-10-22T21:27:54Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:27, 22 October 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size<ins style="font-weight: bold; text-decoration: none;">.Once an SDS-Page Gel electrophoresis has taken place, the fragments of [[DNA|DNA ]]then need to be visualised. This can be done through a few different methods. One way is through UV imaging, another is through adding a dye to the [[DNA|DNA]] fragments</ins>. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

110254403 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=6301&oldid=prev 110339939 at 09:32, 22 October 2012 2012-10-22T09:32:38Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:32, 22 October 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] <del style="font-weight: bold; text-decoration: none;">due to </del>their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]<ins style="font-weight: bold; text-decoration: none;">&nbsp;by&nbsp;</ins>their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

110339939 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=4175&oldid=prev 101442394 at 14:49, 25 November 2011 2011-11-25T14:49:02Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:49, 25 November 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are <del style="font-weight: bold; text-decoration: none;">larher </del>in size.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are <ins style="font-weight: bold; text-decoration: none;">larger </ins>in size. </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

101442394 https://teaching.ncl.ac.uk/bms/wiki//index.php?title=SDS_polyacrylamide-gel_electrophoresis&diff=4174&oldid=prev 101442394 at 14:48, 25 November 2011 2011-11-25T14:48:47Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:48, 25 November 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[<del style="font-weight: bold; text-decoration: none;">polyacrylamide</del>|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[<ins style="font-weight: bold; text-decoration: none;">Polyacrylamide</ins>|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref><ins style="font-weight: bold; text-decoration: none;">. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larher in size</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== References ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references /></div></td></tr> </table>

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