Thermus aquaticus - Revision history

Nnjm2: Cleaned up the page.

2017-10-20T07:00:00Z

Cleaned up the page.

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	''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at ~~supraoptimal~~ temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supra-optimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.

	The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>.		The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>.

	The genome of Thermus aquaticus ~~encompases~~ one chromosome, along with four [[Plasmids\|plasmids]], ~~all together~~ giving a total of over 2mb of genetic content, containing many [[Crispr\|CRISPR ]]~~regions~~. Another interesting feature of the Thermus aquaticus genome is the fact that regions typically found on the ~~megaplasid~~ of a bacterial genome are ~~surprisngly~~ located on the main [[Chromosome\|chromosome ]]of this species, which also comprises partial prophage regions, which show sequence similarity to Thermus species 2.9 <ref>Brumm PJ, Monsma S, Keough B, Jasinovica S, Ferguson E, Schoenfeld T, Lodes M and Mead DA. Complete Genome Sequence of Thermus aquaticus Y51MC23. PLOS ONE. 2015;10.</ref>.  ~~ ~~		The genome of Thermus aquaticus encompasses one chromosome, along with four [[Plasmids\|plasmids]], altogether giving a total of over 2mb of genetic content, containing many [[Crispr\|CRISPR regions]]. Another interesting feature of the Thermus aquaticus genome is the fact that regions typically found on the megaplasmid of a bacterial genome are surprisingly located on the main [[Chromosome\|chromosome of]] this species, which also comprises partial prophage regions, which show sequence similarity to Thermus species 2.9 <ref>Brumm PJ, Monsma S, Keough B, Jasinovica S, Ferguson E, Schoenfeld T, Lodes M and Mead DA. Complete Genome Sequence of Thermus aquaticus Y51MC23. PLOS ONE. 2015;10.</ref>. <br>

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	=== References ===		=== References ===

	<references />~~ . ~~		<references />

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	The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>.		The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>.

			The genome of Thermus aquaticus encompases one chromosome, along with four [[Plasmids\|plasmids]], all together giving a total of over 2mb of genetic content, containing many [[Crispr\|CRISPR ]]regions. Another interesting feature of the Thermus aquaticus genome is the fact that regions typically found on the megaplasid of a bacterial genome are surprisngly located on the main [[Chromosome\|chromosome ]]of this species, which also comprises partial prophage regions, which show sequence similarity to Thermus species 2.9 <ref>Brumm PJ, Monsma S, Keough B, Jasinovica S, Ferguson E, Schoenfeld T, Lodes M and Mead DA. Complete Genome Sequence of Thermus aquaticus Y51MC23. PLOS ONE. 2015;10.</ref>.

			<br>

	=== References ===		=== References ===

	<references />		<references /> .

Nnjm2 at 09:36, 27 October 2015

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← Older revision		Revision as of 09:36, 27 October 2015
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	''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.

	The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors.		The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>.

	=== References ===		=== References ===
	<references />~~<br>~~
	~~3. Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].~~		<references />

140282603 at 14:10, 23 October 2015

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	''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.

	The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and ~~thrives~~ at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors.		The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors.

	=== References ===		=== References ===

	<references /><br>		<references /><br>
			3. Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].

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	''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.

			The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and thrives at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors.

	=== References ===		=== References ===

	<references /><br>		<references /><br>

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	''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[~~Gram_negative~~\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[~~Hydrogen_bonds~~\|hydrogen bonds]] during PCR and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds\|hydrogen bonds]] during [[PCR\|PCR]] and will not denature.

	=== References ===		=== References ===

	<references /><br>		<references /><br>

Nnjm2 at 15:55, 12 November 2011

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	Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California<ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures<ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>.This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.		''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram_negative\|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen_bonds\|hydrogen bonds]] during PCR and will not denature.

	~~<br>~~		=== References ===

	<references /><br>		<references /><br>

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	~~ ~~Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California<ref ~~name="1"~~>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures<ref ~~name="1"~~>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>.This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[~~Taq_polymerase~~\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.		Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California<ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures<ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>.This bacterium has revolutionised the [[PCR\|PCR]] technique as from it, the enzyme [[Taq polymerase\|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.

	<~~ref name="1"~~>~~Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97~~</~~ref~~>		<br>

			<references /><br>

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2011-11-12T14:19:34Z

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 Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California<ref name="1">Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures<ref name="1">Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>.This bacterium has revolutionised the [[PCR|PCR]] technique as from it, the enzyme [[Taq_polymerase|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.

<ref name="1">Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>