Dna isolation: Difference between revisions
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Revision as of 15:20, 19 October 2015
Step 1- Isolate DNA out of the cell
Step 2 - Centrifuge down the fragments at low rpm
Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chloroform
Step 4 - Concentrate DNA using 2 volumes of ethanol
Step 5 - Confirm DNA purity - A260/A280 > 1.8 indicates pure DNA
Principles and techniques of biochemistry and molecular biology, 7th Edition
Wilson K, Walker J
Cambridge University Press, 2009