DNA Taq polymerase: Difference between revisions
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DNA Taq polymerase is an enzyme which is commonly used in the process of PCR. This particular DNA polymerase was isolated from Thermus aquaticus in hot springs <ref>Yamagami, Matsukawa, Tsunekawa, Kawarabayasi, Ishino, Ishino. Gene. (2015)A longer finger-subdomain of family a DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities.</ref>. It is used as it can withstand the high temperatures which are used during the PCR cycle. The role of DNA Taq polymerase is to synthesise DNA from a single stranded DNA template in the presence of dNTPs and a primer. | DNA Taq polymerase is an enzyme which is commonly used in the process of [[PCR|PCR]]. This particular DNA polymerase was isolated from ''[[Thermus_aquaticus|Thermus aquaticus]]'' in hot springs <ref>Yamagami, Matsukawa, Tsunekawa, Kawarabayasi, Ishino, Ishino. Gene. (2015)A longer finger-subdomain of family a DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities.</ref>. It is used as it can withstand the high temperatures which are used during the [[Polymerase_Chain_Reaction_(PCR)|PCR cycle]]. The role of DNA Taq polymerase is to synthesise DNA from a single stranded DNA template in the presence of dNTPs and a [[primer|primer]]. | ||
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Latest revision as of 20:53, 20 October 2015
DNA Taq polymerase is an enzyme which is commonly used in the process of PCR. This particular DNA polymerase was isolated from Thermus aquaticus in hot springs [1]. It is used as it can withstand the high temperatures which are used during the PCR cycle. The role of DNA Taq polymerase is to synthesise DNA from a single stranded DNA template in the presence of dNTPs and a primer.
References
- ↑ Yamagami, Matsukawa, Tsunekawa, Kawarabayasi, Ishino, Ishino. Gene. (2015)A longer finger-subdomain of family a DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities.