Michaelis constant: Difference between revisions

Revision as of 19:34, 30 November 2015

K_m = Michaelis constant

K_m = the value of [S] that causes V= ½ V_max *

The units of K_m are M, concentration

K_m indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.

High K_m demonstrates a low affinity; Low Km demonstrates a high affinity.

Velocity is related to K_mthrough the Michaelis & Menten equation: v = (V_max [S])/(K_m + [S])

V_max = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration

[S] = substrate concentration

@@ Line 1: / Line 1: @@
 K<sub>m</sub> = Michaelis constant
 K<sub>m</sub> = the value of [S] that causes V= ½ V<sub>max</sub> *
@@ Line 5: / Line 5: @@
 The units of K<sub>m</sub> are M, concentration
-K<sub>m</sub> indicates the affinity of the enzyme for it's substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]].
+K<sub>m</sub> indicates the affinity of the enzyme for its substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]].
 High K<sub>m</sub> demonstrates a low affinity; Low Km demonstrates a high affinity.
-velocity is related to K<sub>m&nbsp;</sub>through the Michaelis &amp; Menten equation:&nbsp;v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S])<br>
+Velocity is related to K<sub>m&nbsp;</sub>through the Michaelis &amp; Menten equation:&nbsp;v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S])<br>
 *V<sub>max</sub> = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration
 [S] = substrate concentration<br>