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| It is a powerful method of spreading [[Abc_transporter_protein|protein ]]along a tubular gel using iso-electric focusing and sodium dodecyl sulphate polyacrylamide gel. the sample undergoes [[Iso-electric_focussing|iso-electric focusing ]]first, producing first dimension. the sample is then placed horizontally on top of [[SDS-PAGE|SDS-PAGE ]]and spreads across. the sample then moves vertically down again to yield the second dimension; producing a 2D pattern. So the sample has been separated by virtue of [[Isoelectric_Point|Iso-electric point]] (pl), when they move horizontally and by virtue of their size; when they move down vertically.
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| <ref>Berg,J. stryer, L Tymoczko, J (2011) Biochemistry, 7th Edition, New York: W.H Freenman and Company . Chapter3 page 76.</ref>
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Latest revision as of 15:17, 18 October 2016