Morpholino: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
'''Description:''' | == '''Description:''' == | ||
* Small oligomer of synthetic nucleotide analogues that block gene expression | * Small oligomer of synthetic nucleotide analogues that block gene expression | ||
*25bp length | *25bp length | ||
'''Function:''' | == '''Function:''' == | ||
*attaches to mRNA, pre/mature, to cause gene knockdown | *attaches to mRNA, pre/mature, to cause gene knockdown | ||
| Line 17: | Line 17: | ||
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | *sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | ||
'''References | == '''References''' == | ||
<references /> | == <references /> == | ||
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br> | Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br> | ||
< | <br> | ||
Revision as of 12:29, 16 November 2017
Description:
- Small oligomer of synthetic nucleotide analogues that block gene expression
- 25bp length
Function:
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool on zebra fish
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein
References
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.