Michaelis constant: Difference between revisions
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K<sub>m</sub> = the value of [S] that causes V= ½ V<sub>max</sub> * | K<sub>m</sub> = the value of [S] that causes V= ½ V<sub>max</sub> * | ||
The units of K<sub>m</sub> are M, concentration | The units of K<sub>m</sub> are M, [[Concentration|concentration]]. | ||
K<sub>m</sub> indicates the affinity of the enzyme for its substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]]. | K<sub>m</sub> indicates the [[javascript:void(0);/*1512127072657*/|affinity]] of the enzyme for its substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]]. | ||
High K<sub>m</sub> demonstrates a low affinity; Low Km demonstrates a high affinity. | High K<sub>m</sub> demonstrates a low affinity; Low Km demonstrates a high affinity. | ||
Velocity is related to K<sub>m </sub>through the Michaelis & Menten equation: v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S])<br> | Velocity is related to K<sub>m </sub>through the Michaelis & Menten equation: v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S]).<br> | ||
*V<sub>max</sub> = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration | *[[Vmax|V<sub>max</sub>]] = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration. | ||
[S] = substrate concentration<br> | [S] = [[Substrate|substrate]] concentration.<br> | ||
Revision as of 11:16, 1 December 2017
Km = Michaelis constant
Km = the value of [S] that causes V= ½ Vmax *
The units of Km are M, concentration.
Km indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.
High Km demonstrates a low affinity; Low Km demonstrates a high affinity.
Velocity is related to Km through the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S]).
- Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration.
[S] = substrate concentration.