Pfu: Difference between revisions
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Created page with "<h1> Pfu </h1> <p>Pfu polymerase is an enzyme isolated from the organism Pyrococcus furiosus, a hyperthermophilic archaeon. This organism can survive temperatures of up to o..." |
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Revision as of 14:57, 5 November 2011
Pfu
Pfu polymerase is an enzyme isolated from the organism Pyrococcus furiosus, a hyperthermophilic archaeon. This organism can survive temperatures of up to one hundred degrees celcius, therefore is very apt for use in the <a href="PCR">PCR</a> reaction, as, unlike <a href="DNA polymerase">DNA polymerase</a>, it can survive and function at the high temperatures the process operates at. It may be preferred to the use of <a href="Taq polymerase">Taq polymerase </a>(another thermostable enzyme) as pfu has 3'-5' proof reading activity, improving the accuracy of copying <a href="DNA">DNA</a>.