SDS polyacrylamide-gel electrophoresis: Difference between revisions
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[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of [[Acrylamide|acrylamide]] subunits ([[ | [[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]] due to their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of [[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larher in size. | ||
=== References === | === References === | ||
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Revision as of 14:48, 25 November 2011
SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied [1]. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larher in size.
References
- ↑ Alberts,Bruce et al., 2008 Pg 517