Non-denaturing gel: Difference between revisions
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Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded, however separation does not occur as predictibly as those run in a denaturing gel. | Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded<ref>http://www.ap-lab.com/native_gels.htm</ref>, however separation does not occur as predictibly as those run in a denaturing gel. | ||
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Revision as of 15:07, 26 November 2011
Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrilamide gel electrophoresis (PAGE). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded[1], however separation does not occur as predictibly as those run in a denaturing gel.