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| DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or Okazaki fragments. It works by repairing breaks in the [[Sugar phosphate backbone|sugar phosphate backbone]] in [[DNA|DNA]] by creating a covalent bond between adjoining [[Nucleotides|nucleotides]] (between the 3’hydroxyl group of one DNA molecule with the 5’phosphoryl group of another).<ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones and Bartlett learning (Chapter 2 DNA Structure and Genetic Variation) p440-42</ref><br>In [[Eukaryotes|eukaryotes]] [[DNA ligase I|DNA ligase I]] family members play the major role.The enzyme has a fundamental role in genetic engineering such as recombinant plasmid formation or Polymerase Chain Reaction.<ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones and Bartlett learning (Chapter 2 DNA Structure and Genetic Variation) p440-42</ref> <br>
| | See [[DNA_ligase|DNA Ligase]] |
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| === References ===
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Latest revision as of 18:33, 21 October 2012