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| DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or [[Okazaki fragments|Okazaki fragments]]. It works by repairing breaks in the [[Sugar phosphate backbone|sugar phosphate backbone]] in [[DNA|DNA]] by creating a covalent bond between adjoining [[Nucleotides|nucleotides]] (between the 3’[[Hydroxyl group|hydroxyl group]] of one DNA molecule with the 5’[[Phosphoryl group|phosphoryl group]] of another).<ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones and Bartlett learning (Chapter 2 DNA Structure and Genetic Variation) p440-42</ref><br>In [[Eukaryotes|eukaryotes]] [[DNA ligase I|DNA ligase I]] family members play the major role.The enzyme has a fundamental role in [[Recombinant DNA Technology|genetic engineering]] such as[[Recombinant DNA Technology|recombinant plasmid formation]] or [[PCR|Polymerase Chain Reaction]].<ref>Berg, J.M., Tymoczko, J.L. and Stryer, L. (2012). Biochemistry, 7 th Edition, New York , W.H.Freeman &amp;amp; Co Ltd. p152-153</ref> <br>
| | See [[DNA_ligase|DNA Ligase]] |
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| === References ===
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Latest revision as of 18:33, 21 October 2012