Expect value: Difference between revisions

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 The Expect value (E) is a parameter that describes the number of hits one can "expect" to see by chance when searching a database of a particular size. It decreases exponentially as the Score (S) of the match increases. Essentially, the E value describes the random background noise. For example, an E value of 1 assigned to a hit can be interpreted as meaning that in a database of the current size one might expect to see 1 match with a similar score simply by chance.
The Expect Value (E) is the number of hits that a person would expect by chance when searching a database. The lower this value is, the higher the Score (S) of the match is. The closer the E-value gets to zero, the better the match is


The lower the E-value, or the closer it is to zero, the more "significant" the match is. However, keep in mind that virtually identical short alignments have relatively high E values. This is because the calculation of the E value takes into account the length of the query sequence. These high E values make sense because shorter sequences have a higher probability of occurring in the database purely by chance.
The lower the E-value, or the closer it is to zero, the more "significant" the match is. But another factor that affects the E-value is the length of query. Since shorter ones have a higher chance of occurring in the database, they tend to have a higher E-value.<ref>National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]</ref>
 
The Expect value can also be used as a convenient way to create a significance threshold for reporting results. You can change the Expect value threshold on most BLAST search pages. When the Expect value is increased from the default value of 10, a larger list with more low-scoring hits can be reported.<ref>National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]</ref>  


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'''REFERENCES'''<br> <references />  
'''REFERENCES'''<br> <references />  


<ref>National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]</ref>
<ref>National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]</ref>

Revision as of 20:36, 20 October 2013

The Expect Value (E) is the number of hits that a person would expect by chance when searching a database. The lower this value is, the higher the Score (S) of the match is. The closer the E-value gets to zero, the better the match is

The lower the E-value, or the closer it is to zero, the more "significant" the match is. But another factor that affects the E-value is the length of query. Since shorter ones have a higher chance of occurring in the database, they tend to have a higher E-value.[1]







REFERENCES

  1. National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]

[1]

  1. National Library of Medicine, BLAST Frequently Asked Questions [online], available at: &amp;amp;amp;amp;amp;amp;lt;http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&amp;amp;amp;amp;amp;amp;amp;PAGE_TYPE=BlastDocs&amp;amp;amp;amp;amp;amp;amp;DOC_TYPE=FAQ#expect&amp;amp;amp;amp;amp;amp;gt;, [accessed 20/Oct/2013]