Tween 20: Difference between revisions
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Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting <ref name="S1">Sigma-Aldrich (n.d). Tween 20. | Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting <ref name="S1">Sigma-Aldrich (n.d). Tween 20.fckLRfckLRfckLRhttp://www.sigmaaldrich.com/catalog/product/sigma/p9416?lang=en&amp;amp;amp;amp;amp;amp;amp;amp;amp;region=GB. [Last accessed 20/11/13].</ref>. Other uses of Tween 20 include expulsion of membrane peripheral proteins and lysis of mammalian cells<ref name="S1" />. | ||
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Particularly is Western Blotting, Tween 20 is used to decrease the possibility of unspecific binding of proteins (such as immunoassays) onto the membrane<ref name="B1" />. This reduces the hinderance imposed on the target protein of interest by reducing the background noise (unnecessary binding) <ref name="B2">Galva, C., Gatto, C., Milanick, M. (2012). Soymilk: An effective and inexpensive blocking agent for immunoblotting. Available at http://www.sciencedirect.com/science/article/pii/S0003269712002023. [Last accessed 20/11/13].</ref>. Other potent blocking agents include nonfat soymilk, bovine serum albumin and casein <ref name="B3" />. | |||
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Revision as of 22:34, 20 November 2013
Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting [1]. Other uses of Tween 20 include expulsion of membrane peripheral proteins and lysis of mammalian cells[1].
Particularly is Western Blotting, Tween 20 is used to decrease the possibility of unspecific binding of proteins (such as immunoassays) onto the membrane[2]. This reduces the hinderance imposed on the target protein of interest by reducing the background noise (unnecessary binding) [3]. Other potent blocking agents include nonfat soymilk, bovine serum albumin and casein [4].
- ↑ 1.0 1.1 Sigma-Aldrich (n.d). Tween 20.fckLRfckLRfckLRhttp://www.sigmaaldrich.com/catalog/product/sigma/p9416?lang=en&amp;amp;amp;amp;amp;amp;amp;amp;region=GB. [Last accessed 20/11/13].
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedB1 - ↑ Galva, C., Gatto, C., Milanick, M. (2012). Soymilk: An effective and inexpensive blocking agent for immunoblotting. Available at http://www.sciencedirect.com/science/article/pii/S0003269712002023. [Last accessed 20/11/13].
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedB3