Tween 20: Difference between revisions
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Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting <ref name="A">Sigma-Aldrich (n.d). Tween 20. Available at: | Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent [[Nonionic detergent|nonionic detergent]], emulsifier and as a blocking agent in [[Immunoassay|immunoassays]], such as [[Western Blotting|Western Blotting]] <ref name="A">Sigma-Aldrich (n.d). Tween 20. Available at: http://www.sigmaaldrich.com/catalog/product/sigma/p9416 [Last accessed 20/11/13].</ref>. Other uses of Tween 20 include expulsion of membrane peripheral [[Protein|proteins]] and lysis of mammalian cells<ref name="A" />. | ||
< | Particularly is Western Blotting, Tween 20 is used to decrease the possibility of unspecific binding of proteins (such as immunoassays) onto the nitrocellulose/nylon blotting membrane<ref name="B">Galva, C., Gatto, C., Milanick, M. (2012). Soymilk: An effective and inexpensive blocking agent for immunoblotting. Available at: http://www.sciencedirect.com/science/article/pii/S0003269712002023. [Last accessed 20/11/13].</ref>. This improves visualisation of the protein of interest by reducing the background noise (binding of primary and/or secodary [[Antibody|antibody]] to the membrane)<ref name="B" />. | ||
Other potent blocking agents include nonfat dry milk, bovine serum albumin, casein and possibly nonfat soymilk<ref name="B" />. | |||
=== References === | |||
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Latest revision as of 00:35, 21 November 2013
Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting [1]. Other uses of Tween 20 include expulsion of membrane peripheral proteins and lysis of mammalian cells[1].
Particularly is Western Blotting, Tween 20 is used to decrease the possibility of unspecific binding of proteins (such as immunoassays) onto the nitrocellulose/nylon blotting membrane[2]. This improves visualisation of the protein of interest by reducing the background noise (binding of primary and/or secodary antibody to the membrane)[2].
Other potent blocking agents include nonfat dry milk, bovine serum albumin, casein and possibly nonfat soymilk[2].
References
- ↑ 1.0 1.1 Sigma-Aldrich (n.d). Tween 20. Available at: http://www.sigmaaldrich.com/catalog/product/sigma/p9416 [Last accessed 20/11/13].
- ↑ 2.0 2.1 2.2 Galva, C., Gatto, C., Milanick, M. (2012). Soymilk: An effective and inexpensive blocking agent for immunoblotting. Available at: http://www.sciencedirect.com/science/article/pii/S0003269712002023. [Last accessed 20/11/13].