Taq Polymerase: Difference between revisions

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Taq polymerase is an [[Enzyme|enzyme]] isolated from the bacterium- ''Thermus aquaticus''. Its main use is in [[PCR|PCR]] reactions where its resistance to damage from high temperatures is essential. 
See [[Taq_polymerase|Taq polymerase]]
 
During [[PCR|PCR]], Taq pol is used to extend the [[Primers|primers]] attached to the single stranded [[DNA|DNA]] and therefore create new pieces of DNA complementary to the original sample. This step is repeated 30 times at a temperature of 72 degrees to produce an amplified sample of  the DNA which at its increased volume, is ideal for DNA sequencing.
 
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Latest revision as of 09:21, 20 October 2012