Thermus aquaticus: Difference between revisions
Created page with " Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California<ref name="1">Brock TD, Freeze H. (1969 Apr) The..." |
Cleaned up the page. |
||
| (7 intermediate revisions by 5 users not shown) | |||
| Line 1: | Line 1: | ||
''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supra-optimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR|PCR]] technique as from it, the enzyme [[Taq polymerase|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds|hydrogen bonds]] during [[PCR|PCR]] and will not denature. | |||
<ref | The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors<ref>Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].</ref>. | ||
The genome of Thermus aquaticus encompasses one chromosome, along with four [[Plasmids|plasmids]], altogether giving a total of over 2mb of genetic content, containing many [[Crispr|CRISPR regions]]. Another interesting feature of the Thermus aquaticus genome is the fact that regions typically found on the megaplasmid of a bacterial genome are surprisingly located on the main [[Chromosome|chromosome of]] this species, which also comprises partial prophage regions, which show sequence similarity to Thermus species 2.9 <ref>Brumm PJ, Monsma S, Keough B, Jasinovica S, Ferguson E, Schoenfeld T, Lodes M and Mead DA. Complete Genome Sequence of Thermus aquaticus Y51MC23. PLOS ONE. 2015;10.</ref>. <br> | |||
=== References === | |||
<references /> | |||
Latest revision as of 07:00, 20 October 2017
Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California [1]. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supra-optimal temperatures [2]. This bacterium has revolutionised the PCR technique as from it, the enzyme Taq polymerase has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.
The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and growth conditions thrive at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors[3].
The genome of Thermus aquaticus encompasses one chromosome, along with four plasmids, altogether giving a total of over 2mb of genetic content, containing many CRISPR regions. Another interesting feature of the Thermus aquaticus genome is the fact that regions typically found on the megaplasmid of a bacterial genome are surprisingly located on the main chromosome of this species, which also comprises partial prophage regions, which show sequence similarity to Thermus species 2.9 [4].
References
- ↑ Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97
- ↑ Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97
- ↑ Life at High Temperatures - Thomas D.Brock. 1994.[ONLINE] Available at: http://bioinfo.bact.wisc.edu/themicrobialworld/LAHT/b27.html. [Accessed 1994].
- ↑ Brumm PJ, Monsma S, Keough B, Jasinovica S, Ferguson E, Schoenfeld T, Lodes M and Mead DA. Complete Genome Sequence of Thermus aquaticus Y51MC23. PLOS ONE. 2015;10.