Pyrococcus furiosis: Difference between revisions
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| ''Pyrococcus furiosus ''is an [[hyperthermophilic|hyperthermophilic]] [[Organism|organism]] found in submarine vents, its [[DNA_polymerase|DNA polymerase ]]is thus stable at very high temperatures (thermostable). [[Pfu|Pfu DNA polymerase]] is an archeal DNA [[polymerase|polymerase]] so it is classed as a Family B polymerase. Pfu polymerase is especially useful as it has a 3' to 5' [[proof reading|proof reading]] [[exonuclease|exonuclease]] ability for excision of an incorrect DNA base pair after which the polymerase re-inserts the correct base pair <ref>Edward M. Kennedy, Christopher Hergott, Stephen Dewhurst, and Baek Kim. (2009) The Mechanistic Architecture of the Thermostable Pyrococcus Furiosus Family B DNA Polymerase Motif A and its Interaction with dNTP Substrate. Journal of Biochemistry 48(47): 11161-11168.</ref> . | ||
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Latest revision as of 16:33, 14 November 2011
Pyrococcus furiosus is an hyperthermophilic organism found in submarine vents, its DNA polymerase is thus stable at very high temperatures (thermostable). Pfu DNA polymerase is an archeal DNA polymerase so it is classed as a Family B polymerase. Pfu polymerase is especially useful as it has a 3' to 5' proof reading exonuclease ability for excision of an incorrect DNA base pair after which the polymerase re-inserts the correct base pair [1] .
References
- ↑ Edward M. Kennedy, Christopher Hergott, Stephen Dewhurst, and Baek Kim. (2009) The Mechanistic Architecture of the Thermostable Pyrococcus Furiosus Family B DNA Polymerase Motif A and its Interaction with dNTP Substrate. Journal of Biochemistry 48(47): 11161-11168.