Luciferase: Difference between revisions
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This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the pyrosequencing technique used in new generation DNA sequencing | This a group of [[oxidative enzymes|oxidative enzymes]] that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the [[Pyrosequencing|pyrosequencing]] technique used in new generation [[DNA sequencing|DNA sequencing]] <ref>Gould SJ, Subramani S (November 1988). "Firefly luciferase as a tool in molecular and cell biology". Anal. Biochem. 175 (1): 5–13. doi:10.1016/0003-2697(88)90353-3. PMID 3072883.</ref>. | ||
luciferin + ATP → luciferyl adenylate + PPi<br>luciferyl adenylate + O2 → oxyluciferin + AMP + light<br> | luciferin + ATP → luciferyl adenylate + PPi<br>luciferyl adenylate + O2 → oxyluciferin + AMP + light<br> | ||
=== References === | |||
<references /><br> | |||
Latest revision as of 12:00, 19 October 2012
This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the pyrosequencing technique used in new generation DNA sequencing [1].
luciferin + ATP → luciferyl adenylate + PPi
luciferyl adenylate + O2 → oxyluciferin + AMP + light
References
- ↑ Gould SJ, Subramani S (November 1988). "Firefly luciferase as a tool in molecular and cell biology". Anal. Biochem. 175 (1): 5–13. doi:10.1016/0003-2697(88)90353-3. PMID 3072883.