Ion exchange chromatography: Difference between revisions
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Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition) | Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition). | ||
Revision as of 16:13, 19 November 2010
Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition).