Ion exchange chromatography: Difference between revisions
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Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition). | Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition). | ||
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Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman<br> | |||
Revision as of 16:14, 19 November 2010
Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge. For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column. (Stryer 6th edition).
Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman