Dna isolation: Difference between revisions
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Created page with "Step 1- Isolate DNA out of the cell Step 2 - Centrifuge down the fragments at low rpm Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chlor..." |
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Step 1- Isolate DNA out of the cell | Step 1- Isolate DNA out of the cell | ||
Step 2 - Centrifuge down the fragments at low rpm | Step 2 - Centrifuge down the fragments at low rpm | ||
Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chloroform | Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chloroform | ||
Step 4 - Concentrate DNA using 2 volumes of ethanol | Step 4 - Concentrate DNA using 2 volumes of ethanol | ||
Step 5 - Confirm DNA purity - A260/A280 > 1.8 indicates pure DNA | Step 5 - Confirm DNA purity - A260/A280 > 1.8 indicates pure DNA | ||
Principles and techniques of biochemistry and molecular biology, 7th Edition | <ref>Principles and techniques of biochemistry and molecular biology, 7th Edition Wilson K, Walker J Cambridge University Press, 2009</ref> | ||
Revision as of 15:22, 19 October 2015
Step 1- Isolate DNA out of the cell
Step 2 - Centrifuge down the fragments at low rpm
Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chloroform
Step 4 - Concentrate DNA using 2 volumes of ethanol
Step 5 - Confirm DNA purity - A260/A280 > 1.8 indicates pure DNA
- ↑ Principles and techniques of biochemistry and molecular biology, 7th Edition Wilson K, Walker J Cambridge University Press, 2009