Morpholino: Difference between revisions
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== '''Description:''' == | == '''Description:''' == | ||
* | *Small oligomer of synthetic nucleotide analogues that block gene expression | ||
*25bp length | *25bp length | ||
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*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | *sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | ||
<br> | |||
== '''References''' == | == '''References''' == | ||
Revision as of 12:30, 16 November 2017
Description:
- Small oligomer of synthetic nucleotide analogues that block gene expression
- 25bp length
Function:
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool on zebra fish
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein
References
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.