DNA polymerase I: Difference between revisions
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DNA polymerase I is an enzyme that is involved in DNA replication in [[E.Coli| | DNA polymerase I is an enzyme that is involved in DNA replication in [[E.Coli|E.coli]]. It is part of a group of [[DNA_polymerase|DNA polymerases]] that copy DNA; they are highly coordinated in order to replicate whole genomes precisely and rapidly. DNA polymerase I is a slow, non-processive enzyme that fills the gaps between [[Okazaki_fragment|Okazaki]] fragments of the lagging strand by Watson-Crick base pairing. The enzyme has two additional activites: | ||
1) 5’→3’ exonuclease activity to remove RNA primers ahead of the polymerase site<ref>Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801</ref>.<br>2) 3’→5’ exonuclease activity for proof-reading and accuracy. | 1) 5’→3’ exonuclease activity to remove RNA primers ahead of the polymerase site<ref>Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801</ref>.<br>2) 3’→5’ exonuclease activity for proof-reading and accuracy. | ||
=== References | === References === | ||
<references /> | <references /> | ||
Revision as of 22:19, 27 November 2010
DNA polymerase I is an enzyme that is involved in DNA replication in E.coli. It is part of a group of DNA polymerases that copy DNA; they are highly coordinated in order to replicate whole genomes precisely and rapidly. DNA polymerase I is a slow, non-processive enzyme that fills the gaps between Okazaki fragments of the lagging strand by Watson-Crick base pairing. The enzyme has two additional activites:
1) 5’→3’ exonuclease activity to remove RNA primers ahead of the polymerase site[1].
2) 3’→5’ exonuclease activity for proof-reading and accuracy.
References
- ↑ Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801