PuC18: Difference between revisions
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Furthermore, the gene for [[Ampicillin|ampicillin]] [[Antibiotic resistance|antibiotic resistance]], amp<sup>R</sup>, is also a major feature fo puC18 alongside the main [[Origin of replication|origin of replication]], ori<sup>C</sup>. | Furthermore, the gene for [[Ampicillin|ampicillin]] [[Antibiotic resistance|antibiotic resistance]], amp<sup>R</sup>, is also a major feature fo puC18 alongside the main [[Origin of replication|origin of replication]], ori<sup>C</sup>. | ||
Overall, the [[Vector|expression vector]] is 2686 base pairs in total and has a [[Molecular weight|molecular weight]] of 1.75x10<sup>6</sup> Da. | Overall, the [[Vector|expression vector]] is 2686 base pairs in total and has a [[Molecular weight|molecular weight]] of 1.75x10<sup>6</sup> Da<ref>reference</ref>. | ||
1. pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/ | |||
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Revision as of 21:11, 2 December 2017
PuC18 is a common bacterial plasmid for E. Coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination.
Furthermore, the gene for ampicillin antibiotic resistance, ampR, is also a major feature fo puC18 alongside the main origin of replication, oriC.
Overall, the expression vector is 2686 base pairs in total and has a molecular weight of 1.75x106 Da[1].
1. pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/
- ↑ reference