ELISA: Difference between revisions
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Enzyme-Linked Immunosorbent Assay (ELISA) is a technique whereby antibodies are used for detecting and quantifying the prescence of a specific protein or antigen. The antibodies used in the technique have been modified such that they are linked with an enzyme that reacts with a particular substrate and results in a change in the colour of that substrate. The technique relies on the formation of an antibody-antigen complex when the protein comes in contact with the antibody within the reagent. | Enzyme-Linked Immunosorbent Assay (ELISA) is a technique whereby [[antibody|antibodies]] are used for detecting and quantifying the prescence of a specific [[protein|protein]] or [[Antigen|antigen]]. The antibodies used in the technique have been modified such that they are linked with an [[enzyme|enzyme]] that reacts with a particular substrate and results in a change in the colour of that substrate. The technique relies on the formation of an [[antibody-antigen complex|antibody-antigen complex]] when the protein comes in contact with the antibody within the reagent. | ||
Revision as of 18:28, 21 October 2012
Enzyme-Linked Immunosorbent Assay (ELISA) is a technique whereby antibodies are used for detecting and quantifying the prescence of a specific protein or antigen. The antibodies used in the technique have been modified such that they are linked with an enzyme that reacts with a particular substrate and results in a change in the colour of that substrate. The technique relies on the formation of an antibody-antigen complex when the protein comes in contact with the antibody within the reagent.