Michaelis constant: Difference between revisions
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*Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration | *Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration | ||
[S] = substrate concentration | [S] = substrate concentration | ||
Revision as of 12:46, 29 November 2012
Km = Michaelis constant
Km = the value of [S] that causes V= ½ Vmax *
The units of Km are M, concentration
Km indicates the affinity of the enzyme for it's substrate and thus the stability of the Enzyme-Substrate Complex.
High Km demonstrates a low affinity; Low Km demonstrates a high affinity.
velocity is related to Km through the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S])
References:
- Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration
[S] = substrate concentration
- ↑ Lakey, J. 2012. BGM1002 Lectures 6&7&8: Enzymes. [lecture]. October 15. Newcastle: DSLT