Transgenic animal: Difference between revisions
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A transgenic animal contains a foreign [[gene|gene]] or an inactive endogenous gene. The foreign DNA is introduced at the single cell stage, it is usually injected into [[stem cells|stem cells]] taken from a [[Blastocyst|blastocyst]]. | |||
There are 2 common methods for creating a transgenic animal the first is microinjection and the second is gene knockouts. | There are 2 common methods for creating a transgenic animal the first is microinjection and the second is gene knockouts. | ||
Mircoinjection only has a 2% sucess rate and 99.9% of the trasngenic results arise due to random intergration not homologous recombination. This methos is not appropriate for generating null replacements. | Mircoinjection only has a 2% sucess rate and 99.9% of the trasngenic results arise due to random intergration not [[Homologous recombination|homologous recombination]]. This methos is not appropriate for generating null replacements. | ||
The gene knockout method involves cloning the gene you wish to knockout; inserting a selectoin marker i.e an antibiotic resistance gene; introducing the mutated gene into the embryonic cells and then selecting for a homologous recombinant. | The gene knockout method involves cloning the gene you wish to knockout; inserting a selectoin marker i.e. an antibiotic resistance gene; introducing the mutated gene into the [[embryonic cells|embryonic cells]] and then selecting for a homologous recombinant <ref>http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html</ref>. | ||
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Latest revision as of 15:45, 16 October 2014
A transgenic animal contains a foreign gene or an inactive endogenous gene. The foreign DNA is introduced at the single cell stage, it is usually injected into stem cells taken from a blastocyst.
There are 2 common methods for creating a transgenic animal the first is microinjection and the second is gene knockouts.
Mircoinjection only has a 2% sucess rate and 99.9% of the trasngenic results arise due to random intergration not homologous recombination. This methos is not appropriate for generating null replacements.
The gene knockout method involves cloning the gene you wish to knockout; inserting a selectoin marker i.e. an antibiotic resistance gene; introducing the mutated gene into the embryonic cells and then selecting for a homologous recombinant [1].