Fluorescence microscope: Difference between revisions
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It is a microscope designed to view material stained with fluorescent dyes<ref>Alberts, B. (2008). Molecular biology of the cell. New York: Garland Science.</ref>. Similar to a light microscope but the illuminating light is passed through one set of filters before the specimen, to select those wavelengths that excite the dye, and through another set of filters before it reches the eye, to select only those wavelengths emitted when the dye fluoresces<ref>Alberts, B. (2008). Molecular biology of the cell. New York: Garland Science.</ref>. | It is a microscope designed to view material stained with [[Fluorescent_dye|fluorescent]] dyes<ref>Alberts, B. (2008). Molecular biology of the cell. New York: Garland Science.</ref>. Similar to a light microscope but the illuminating light is passed through one set of filters before the specimen, to select those [[Wavelength|wavelengths]] that excite the dye, and through another set of filters before it reches the eye, to select only those wavelengths emitted when the dye fluoresces<ref>Alberts, B. (2008). Molecular biology of the cell. New York: Garland Science.</ref>. | ||
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Revision as of 20:48, 21 October 2014
It is a microscope designed to view material stained with fluorescent dyes[1]. Similar to a light microscope but the illuminating light is passed through one set of filters before the specimen, to select those wavelengths that excite the dye, and through another set of filters before it reches the eye, to select only those wavelengths emitted when the dye fluoresces[2].