Michaelis menten equation: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 7: | Line 7: | ||
'''V'''<sub>'''max'''</sub> is the maximum rate of an [[Enzyme|enzyme]] reaction, occurs when all substrate is saturated.<br> | '''V'''<sub>'''max'''</sub> is the maximum rate of an [[Enzyme|enzyme]] reaction, occurs when all substrate is saturated.<br> | ||
'''K<sub>m</sub>''' is the Michaelis-Menten constant and is the substrate concentration at half V<sub>max </sub>.<br> | '''K<sub>m</sub>''' is the Michaelis-Menten constant and is the substrate concentration at half V<sub>max </sub>. (Higher the Km value, lower the affinity)<br> | ||
<sub></sub>V<sub>max</sub> and K<sub>m</sub> can also be shown on a graph, the graph which shows this best is a '''double reciprocal plot '''([[Lineweaver-Burk|Lineweaver-Burk]] plot). You can obtain the results by plotting 1/V against 1/[S] <ref>Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163</ref>. | <sub></sub>V<sub>max</sub> and K<sub>m</sub> can also be shown on a graph, the graph which shows this best is a '''double reciprocal plot '''([[Lineweaver-Burk|Lineweaver-Burk]] plot). You can obtain the results by plotting 1/V against 1/[S] <ref>Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163</ref>. | ||
Latest revision as of 10:36, 22 October 2014
The Michaelis-Menten equation is used to work out the rate of enzyme reactions and is written as follows:
V = Vmax [S]
Km + [S]
Vmax is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.
Km is the Michaelis-Menten constant and is the substrate concentration at half Vmax . (Higher the Km value, lower the affinity)
Vmax and Km can also be shown on a graph, the graph which shows this best is a double reciprocal plot (Lineweaver-Burk plot). You can obtain the results by plotting 1/V against 1/[S] [1].
References
- ↑ Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163