Pyrosequencing: Difference between revisions

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Pyrosequencing (also known as 454-sequencing) is a method of [[DNA Sequencing|DNA Sequencing]]. It involves using an [[Enzyme|enzyme]] called [[luciferase|luciferase]], amongst others, to determine the [[nucleotide|nucleotide]] sequence based upon the light emitted when a correct [[dNTP|dNTP]] binds to a template.
Pyrosequencing (also known as 454-sequencing) is a method of [[DNA Sequencing|DNA Sequencing]]. It involves using an [[Enzyme|enzyme]] called [[Luciferase|luciferase]], amongst others, to determine the [[Nucleotide|nucleotide]] sequence based upon the light&nbsp;emitted when a correct [[DNTP|dNTP]] binds to a template. <references />
 
 
 
<ref>Ronaghi M. Pyrosequencing Sheds Light On DNA Sequencing, Genome Res. 2001 11: 3-11</ref>

Revision as of 12:50, 19 November 2010

Pyrosequencing (also known as 454-sequencing) is a method of DNA Sequencing. It involves using an enzyme called luciferase, amongst others, to determine the nucleotide sequence based upon the light emitted when a correct dNTP binds to a template.


[1]

  1. Ronaghi M. Pyrosequencing Sheds Light On DNA Sequencing, Genome Res. 2001 11: 3-11