Ion exchange chromatography: Difference between revisions
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Ion-exchange [[Chromatography|chromatography]] separates [[Proteins|proteins]] according to their net charge. The stationary phase of the column will have a specific charge. | |||
< | For example negatively charged [[Proteins|proteins]] ([[Anions|anions]]) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged [[Buffer|buffer]] which will compete with the [[Protein|protein]] for binding to the column. Proteins that have lower charge density will be eluted first from the column <ref>Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman</ref>. | ||
=== References === | |||
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Revision as of 16:30, 19 November 2010
Ion-exchange chromatography separates proteins according to their net charge. The stationary phase of the column will have a specific charge.
For example negatively charged proteins (anions) will bind to a positively charged diethylaminoethylcellulose (DEAE-cellulose) columns. The negative proteins bound to column can by eluted by adding a negatively charged buffer which will compete with the protein for binding to the column. Proteins that have lower charge density will be eluted first from the column [1].
References
- ↑ Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman