Dna isolation: Difference between revisions

Revision as of 15:24, 19 October 2015

Step 1- Isolate DNA out of the cell

Step 2 - Centrifuge down the fragments at low rpm

Step 3 - Separate DNA from proteins and lipids by adding equal volume of phenol/chloroform

Step 4 - Concentrate DNA using 2 volumes of ethanol

Step 5 - Confirm DNA purity - A260/A280 > 1.8 indicates pure DNA ^[1]

↑ <Principles and techniques of biochemistry and molecular biology, 7th Edition Wilson K, Walker J Cambridge University Press, 2009>

@@ Line 7: / Line 7: @@
 Step 4 - Concentrate DNA using 2 volumes of ethanol
-Step 5 - Confirm DNA purity - A260/A280 &gt; 1.8 indicates pure DNA
+Step 5 - Confirm DNA purity - A260/A280 &gt; 1.8 indicates pure DNA <ref>&lt;Principles and techniques of biochemistry and molecular biology, 7th Edition Wilson K, Walker J Cambridge University Press, 2009&gt;</ref>
-&nbsp;<ref>Principles and techniques of biochemistry and molecular biology, 7th Edition Wilson K, Walker J Cambridge University Press, 2009</ref>
+&nbsp;