SDS polyacrylamide-gel electrophoresis: Difference between revisions
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SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied. | SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied.<ref>Alberts,Bruce et al(2008) Molecular Biology of the Cell(5th edition) Garland Science Pg 517</ref> | ||
Revision as of 18:09, 20 November 2010
SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied.[1]
- ↑ Alberts,Bruce et al(2008) Molecular Biology of the Cell(5th edition) Garland Science Pg 517