Thermus aquaticus: Difference between revisions
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''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR|PCR]] technique as from it, the enzyme [[Taq polymerase|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds|hydrogen bonds]] during [[PCR|PCR]] and will not denature. | ''Thermus aquaticus'' is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. Isolates of ''T. aquaticus'' are [[Gram negative|gram-negative]] nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures <ref>Brock TD, Freeze H. (1969 Apr) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 98(1):289-97</ref>. This bacterium has revolutionised the [[PCR|PCR]] technique as from it, the enzyme [[Taq polymerase|Taq polymerase]] has been harvested. Taq polymerase can withstand the high temperatures required to break the [[Hydrogen bonds|hydrogen bonds]] during [[PCR|PCR]] and will not denature. | ||
The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and thrives at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors. | |||
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Revision as of 14:05, 23 October 2015
Thermus aquaticus is a thermophilic bacterium that is isolated from thermal springs in Yellowstone National Park, California [1]. Isolates of T. aquaticus are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures [2]. This bacterium has revolutionised the PCR technique as from it, the enzyme Taq polymerase has been harvested. Taq polymerase can withstand the high temperatures required to break the hydrogen bonds during PCR and will not denature.
The Thermus aquaticus can survive in temperatures ranging from 50°C to 80°C, and thrives at approximately 70°C. The cylindrical bacterium is a chemotroph whereby it gains energy from the oxidation of electron donors.