Morpholino: Difference between revisions
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* Small oligomer of synthetic nucleotide analogues that block gene expression | '''Description:''' | ||
* Small oligomer of synthetic nucleotide analogues that block gene expression | |||
*25bp length | *25bp length | ||
*attaches to mRNA, pre/mature, to cause gene knockdown | |||
'''Function:''' | |||
*attaches to mRNA, pre/mature, to cause gene knockdown | |||
*Used as gene knockdown tool on zebra fish | *Used as gene knockdown tool on zebra fish | ||
On mature mRNA: | On mature mRNA: | ||
*attaches to AUG blocking ribosome interaction | *attaches to AUG blocking ribosome interaction | ||
On pre-mRNA: | On pre-mRNA: | ||
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | *sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br> | ||
<br> | |||
---- | |||
'''References | |||
<references /> | |||
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br> | |||
<references / | <references /> | ||
Revision as of 12:28, 16 November 2017
Description:
- Small oligomer of synthetic nucleotide analogues that block gene expression
- 25bp length
Function:
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool on zebra fish
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein
References
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.