Polyacrylamide: Difference between revisions
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Polyacrylamide is a three-dimensional mesh formed by the copolymerization of an activated monomer [[Acrylamide|acrylamide]] and a cross-linking agent [[Methylenebisacrylamide|methylenebisacrylamide]], initiated by the presence of sulphate free radicals. A polyacrylamide gel is used in [[SDS_polyacrylamide-gel_electrophoresis|SDS Polyacrylamide-Gel Elctrophoresis]] ([[SDS_polyacrylamide-gel_electrophoresis|SDS-PAGE]]), because it is chemically inert so there is no chemical interference with the proteins to be separated, is readily formed by its monomers and the pore size of the gel can be adjusted to separate [[Proteins|proteins]] of different sizes by retarding the [[Proteins|proteins]] of interest <ref>Biochemistry 6th edition pg 71/72, Stryer Lubert, Tymoczko John.L, Berg Jeremy, published by W.H.Freeman and Company, New York (2007)</ref> <ref>Molecular Biology of The Cell 5th edition pg 517, Alberts, Johnson, Lewis, Raff, Roberts and Walter,published by Garland Science, New York (2008)</ref>. | |||
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Latest revision as of 18:28, 28 November 2010
Polyacrylamide is a three-dimensional mesh formed by the copolymerization of an activated monomer acrylamide and a cross-linking agent methylenebisacrylamide, initiated by the presence of sulphate free radicals. A polyacrylamide gel is used in SDS Polyacrylamide-Gel Elctrophoresis (SDS-PAGE), because it is chemically inert so there is no chemical interference with the proteins to be separated, is readily formed by its monomers and the pore size of the gel can be adjusted to separate proteins of different sizes by retarding the proteins of interest [1] [2].
References