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Reverse transcription is the process of transcribing complementary | Reverse transcription is the process of transcribing mRNA into complementary DNA. The enzyme used is reverse transcriptase. Reverse transcriptase will need a short RNA primer called oligonucleotide to start off the process. Primer will bind to the 3-prime poly A tail then reverse transcriptase will add on the nucleotide which are complementary to the mRNA strand synthesizing cDNA from 5'-3' direction.<ref>CliffsNotes.Reverse transcription.nd.Available at:https://www.cliffsnotes.com/study-guides/biology/biochemistry-ii/eukaryotic-genes/reverse-transcription</ref> | ||
<br>Reverse transcription are used by retrovirus. Retrovirus is a virus which contains RNA as their genome. Once retrovirus enters to a host cell, such as human cells, the virus will use their own reverse transcriptase to convert their genome into cDNA. The DNA is then taken in and treat as the host cell's genome. The viral DNA will then be transribed and translated into proteins which is essential for the virus to produce a new copies of virus cells.<ref>Verywell Future.How a retrovirus or RNA virus work 2018.[Cited 16/2/2018] Available at:https://www.verywellhealth.com/hiv-is-a-retrovirus-what-does-that-mean-3132822</ref> | |||
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Revision as of 09:51, 23 November 2018
Reverse transcription is the process of transcribing mRNA into complementary DNA. The enzyme used is reverse transcriptase. Reverse transcriptase will need a short RNA primer called oligonucleotide to start off the process. Primer will bind to the 3-prime poly A tail then reverse transcriptase will add on the nucleotide which are complementary to the mRNA strand synthesizing cDNA from 5'-3' direction.[1]
Reverse transcription are used by retrovirus. Retrovirus is a virus which contains RNA as their genome. Once retrovirus enters to a host cell, such as human cells, the virus will use their own reverse transcriptase to convert their genome into cDNA. The DNA is then taken in and treat as the host cell's genome. The viral DNA will then be transribed and translated into proteins which is essential for the virus to produce a new copies of virus cells.[2]
- ↑ CliffsNotes.Reverse transcription.nd.Available at:https://www.cliffsnotes.com/study-guides/biology/biochemistry-ii/eukaryotic-genes/reverse-transcription
- ↑ Verywell Future.How a retrovirus or RNA virus work 2018.[Cited 16/2/2018] Available at:https://www.verywellhealth.com/hiv-is-a-retrovirus-what-does-that-mean-3132822